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Catalog# Product Description |
ICP9713 Affinity purified Rabbit anti-Human IgG antibodies are developed using purified human IgG as the immunogen. The antibodies are conjugated to horse radish peroxidase (HRP) and are useful for detection, identification, quantization, isolation and localization of human immunoglobulins. |
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Species
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Rabbit
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Formulation
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1 mg/mL in 50% glycerol
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Immunogen
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Pure human IgG (whole molecule)
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Purification
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The antibody was affinity purified with human IgG immobilized on agarose and absorbed with BSA and mouse antibodies.
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Conjugation
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Reductive amination with HRP/Ab ratio - 2:1
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Specificity
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The antibody is specific against human IgG. It does not cross react with human serum albumin or mouse IgG.
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Applications
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ELISA (0.25 - 1.0 μg/mL); WB (0.25 - 1.0 μg/mL); IHC; IP
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Scientific Description
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IgG is a monomeric immunoglobulin. It is built of two heavy chains ? and two light chains. Each IgG has two antigen binding sites that bind with many pathogens including viruses, bacteria and fungi. It also assists in phagocytosis and neutralization of bacterial toxins.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is six months from date of shipping if properly stored.
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References
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1. Junqueira, L. C. et al. Basic Histology, 2003 McGraw-Hill
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